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Year : 2020  |  Volume : 22  |  Issue : 1  |  Page : 62-68

Alpha-lipoic acid attenuates cyclophosphamide-doxorubicin-induced hepatic perturbation in rats

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Niger Delta University, Bayelsa State, Nigeria

Correspondence Address:
Lecturer (Dr) Elias Adikwu
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Niger Delta University, Bayelsa State
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jmms.jmms_64_19

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Background and Objectives: The clinical use of cyclophosphamide-doxorubicin (CP-DOX) in breast cancer treatment may cause hepatotoxicity. This study assessed the protective effect of alpha-lipoic acid (ALA) against hepatotoxicity induced by CP-DOX in albino rats. Materials and Methods: Thirty-six adult male albino rats were randomized into six groups (A-F) of n = 6. Group A (control) was treated intraperitoneally (ip) with 0.3 mL of normal saline 8 hourly for 48h. Group B was treated with 10 mg/kg of ALA 8 hourly ip for 48 h. Group C was treated with a dose of CP-DOX (150/20 mg/kg) for 24 h. Group D was pre-treated with ALA 8 hourly for 48 h before treatment with a dose of CP-DOX ip for 24h. Group E was co-treated with a dose of CP-DOX and ALA ip 8 hourly for 48 h. Group F was treated with a dose of CP-DOX for 24 h before treatment with ALA ip 8 hourly for 48 h. After treatment, rats were euthanized; blood samples were collected and evaluated for serum liver function markers. Liver samples were evaluated for biochemical markers and histology. Results: Liver catalase, superoxide dismutase, glutathione (GSH), and GSH peroxidase levels were significantly (P < 0.001) decreased in CP-DOX-treated rats. Aminotransferases, alkaline phosphatase, gamma glutamyl transferase, lactate dehydrogenase, total bilirubin, conjugated bilirubin, and malondialdehyde levels were significantly (P < 0.001) increased in CP-DOX-treated rats. The liver of CP-DOX-treated rats showed hepatocyte necrosis. However, CP-DOX-induced hepatotoxicity was significantly reversed in rats pre-treated (P < 0.001), co-treated (P < 0.01), and post-treated (P < 0.05) with ALA when compared to CP-DOX-treated rats. Conclusion: Pre-treatment with ALA produced the best protective effect against CP-DOX-induced hepatotoxicity.

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